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primary anti lcn2 antibody  (R&D Systems)
96
R&D Systems primary anti lcn2 antibody
Hemosiderin deposits in ovarian tissues. Formalin-fixated paraffin-embedded ovarian tissues section of wild type (WT, n=8) and Esr1 -deficient (n=8) animals were used for staining. Serial tissue slices of WT (A) and Esr1 -deficient (B) ovaries were stained with Hematoxylin-Eosin (HE) or Perls Prussian Blue (PPB) to detect iron in form of hemosiderin. Hemosiderin can be seen in HE staining as brown-gold deposits and turns blue with PPB. The scale bars equal to 250 µm (solid boarder) or 50 µm (dashed border). Hemosiderin accumulations were found in ovarian stroma of Esr1 -deficient animals but not in WTs and were localized around hemorrhagic cysts (C) . The scale bars equal to 100 µm. (D) Immunohistochemical localization of <t>lipocalin</t> <t>2</t> <t>(LCN2)</t> was combined with PPB staining to examine whether iron-positive cells co-localize with LCN2-positive cells. WT (n=3) and Esr1 -deficient (n=4) ovaries were stained, showing no co-localization of iron and LCN2. Normal goat IgG was used as a negative control instead of the primary antibody. The scale bars equal to 500 µm (solid boarder) or 50 µm (dotted boarder and dashed border).
Primary Anti Lcn2 Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/primary anti lcn2 antibody/product/R&D Systems
Average 96 stars, based on 1 article reviews
primary anti lcn2 antibody - by Bioz Stars, 2026-03
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primary antibodies against lcn2  (Proteintech)
95
Proteintech primary antibodies against lcn2
Hemosiderin deposits in ovarian tissues. Formalin-fixated paraffin-embedded ovarian tissues section of wild type (WT, n=8) and Esr1 -deficient (n=8) animals were used for staining. Serial tissue slices of WT (A) and Esr1 -deficient (B) ovaries were stained with Hematoxylin-Eosin (HE) or Perls Prussian Blue (PPB) to detect iron in form of hemosiderin. Hemosiderin can be seen in HE staining as brown-gold deposits and turns blue with PPB. The scale bars equal to 250 µm (solid boarder) or 50 µm (dashed border). Hemosiderin accumulations were found in ovarian stroma of Esr1 -deficient animals but not in WTs and were localized around hemorrhagic cysts (C) . The scale bars equal to 100 µm. (D) Immunohistochemical localization of <t>lipocalin</t> <t>2</t> <t>(LCN2)</t> was combined with PPB staining to examine whether iron-positive cells co-localize with LCN2-positive cells. WT (n=3) and Esr1 -deficient (n=4) ovaries were stained, showing no co-localization of iron and LCN2. Normal goat IgG was used as a negative control instead of the primary antibody. The scale bars equal to 500 µm (solid boarder) or 50 µm (dotted boarder and dashed border).
Primary Antibodies Against Lcn2, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/primary antibodies against lcn2/product/Proteintech
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primary antibodies against lcn2 - by Bioz Stars, 2026-03
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primary antibodies of rabbit anti-lipocalin-2/neutrophil gelatinase-associated lipocalin (lcn2/ngal)  (Servicebio Inc)
90
Servicebio Inc primary antibodies of rabbit anti-lipocalin-2/neutrophil gelatinase-associated lipocalin (lcn2/ngal)
Hemosiderin deposits in ovarian tissues. Formalin-fixated paraffin-embedded ovarian tissues section of wild type (WT, n=8) and Esr1 -deficient (n=8) animals were used for staining. Serial tissue slices of WT (A) and Esr1 -deficient (B) ovaries were stained with Hematoxylin-Eosin (HE) or Perls Prussian Blue (PPB) to detect iron in form of hemosiderin. Hemosiderin can be seen in HE staining as brown-gold deposits and turns blue with PPB. The scale bars equal to 250 µm (solid boarder) or 50 µm (dashed border). Hemosiderin accumulations were found in ovarian stroma of Esr1 -deficient animals but not in WTs and were localized around hemorrhagic cysts (C) . The scale bars equal to 100 µm. (D) Immunohistochemical localization of <t>lipocalin</t> <t>2</t> <t>(LCN2)</t> was combined with PPB staining to examine whether iron-positive cells co-localize with LCN2-positive cells. WT (n=3) and Esr1 -deficient (n=4) ovaries were stained, showing no co-localization of iron and LCN2. Normal goat IgG was used as a negative control instead of the primary antibody. The scale bars equal to 500 µm (solid boarder) or 50 µm (dotted boarder and dashed border).
Primary Antibodies Of Rabbit Anti Lipocalin 2/Neutrophil Gelatinase Associated Lipocalin (Lcn2/Ngal), supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/primary antibodies of rabbit anti-lipocalin-2/neutrophil gelatinase-associated lipocalin (lcn2/ngal)/product/Servicebio Inc
Average 90 stars, based on 1 article reviews
primary antibodies of rabbit anti-lipocalin-2/neutrophil gelatinase-associated lipocalin (lcn2/ngal) - by Bioz Stars, 2026-03
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primary antibodies against lipocalin-2 (lcn2)  (ABclonal Biotechnology)
90
ABclonal Biotechnology primary antibodies against lipocalin-2 (lcn2)
Hemosiderin deposits in ovarian tissues. Formalin-fixated paraffin-embedded ovarian tissues section of wild type (WT, n=8) and Esr1 -deficient (n=8) animals were used for staining. Serial tissue slices of WT (A) and Esr1 -deficient (B) ovaries were stained with Hematoxylin-Eosin (HE) or Perls Prussian Blue (PPB) to detect iron in form of hemosiderin. Hemosiderin can be seen in HE staining as brown-gold deposits and turns blue with PPB. The scale bars equal to 250 µm (solid boarder) or 50 µm (dashed border). Hemosiderin accumulations were found in ovarian stroma of Esr1 -deficient animals but not in WTs and were localized around hemorrhagic cysts (C) . The scale bars equal to 100 µm. (D) Immunohistochemical localization of <t>lipocalin</t> <t>2</t> <t>(LCN2)</t> was combined with PPB staining to examine whether iron-positive cells co-localize with LCN2-positive cells. WT (n=3) and Esr1 -deficient (n=4) ovaries were stained, showing no co-localization of iron and LCN2. Normal goat IgG was used as a negative control instead of the primary antibody. The scale bars equal to 500 µm (solid boarder) or 50 µm (dotted boarder and dashed border).
Primary Antibodies Against Lipocalin 2 (Lcn2), supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/primary antibodies against lipocalin-2 (lcn2)/product/ABclonal Biotechnology
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primary antibodies against lipocalin-2 (lcn2) - by Bioz Stars, 2026-03
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primary antibodies against lcn2  (Huabio Inc)
90
Huabio Inc primary antibodies against lcn2
Hemosiderin deposits in ovarian tissues. Formalin-fixated paraffin-embedded ovarian tissues section of wild type (WT, n=8) and Esr1 -deficient (n=8) animals were used for staining. Serial tissue slices of WT (A) and Esr1 -deficient (B) ovaries were stained with Hematoxylin-Eosin (HE) or Perls Prussian Blue (PPB) to detect iron in form of hemosiderin. Hemosiderin can be seen in HE staining as brown-gold deposits and turns blue with PPB. The scale bars equal to 250 µm (solid boarder) or 50 µm (dashed border). Hemosiderin accumulations were found in ovarian stroma of Esr1 -deficient animals but not in WTs and were localized around hemorrhagic cysts (C) . The scale bars equal to 100 µm. (D) Immunohistochemical localization of <t>lipocalin</t> <t>2</t> <t>(LCN2)</t> was combined with PPB staining to examine whether iron-positive cells co-localize with LCN2-positive cells. WT (n=3) and Esr1 -deficient (n=4) ovaries were stained, showing no co-localization of iron and LCN2. Normal goat IgG was used as a negative control instead of the primary antibody. The scale bars equal to 500 µm (solid boarder) or 50 µm (dotted boarder and dashed border).
Primary Antibodies Against Lcn2, supplied by Huabio Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/primary antibodies against lcn2/product/Huabio Inc
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primary antibodies against lcn2 - by Bioz Stars, 2026-03
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rat monoclonal primary antibodies to lcn2  (Novus Biologicals)
90
Novus Biologicals rat monoclonal primary antibodies to lcn2
Hemosiderin deposits in ovarian tissues. Formalin-fixated paraffin-embedded ovarian tissues section of wild type (WT, n=8) and Esr1 -deficient (n=8) animals were used for staining. Serial tissue slices of WT (A) and Esr1 -deficient (B) ovaries were stained with Hematoxylin-Eosin (HE) or Perls Prussian Blue (PPB) to detect iron in form of hemosiderin. Hemosiderin can be seen in HE staining as brown-gold deposits and turns blue with PPB. The scale bars equal to 250 µm (solid boarder) or 50 µm (dashed border). Hemosiderin accumulations were found in ovarian stroma of Esr1 -deficient animals but not in WTs and were localized around hemorrhagic cysts (C) . The scale bars equal to 100 µm. (D) Immunohistochemical localization of <t>lipocalin</t> <t>2</t> <t>(LCN2)</t> was combined with PPB staining to examine whether iron-positive cells co-localize with LCN2-positive cells. WT (n=3) and Esr1 -deficient (n=4) ovaries were stained, showing no co-localization of iron and LCN2. Normal goat IgG was used as a negative control instead of the primary antibody. The scale bars equal to 500 µm (solid boarder) or 50 µm (dotted boarder and dashed border).
Rat Monoclonal Primary Antibodies To Lcn2, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rat monoclonal primary antibodies to lcn2/product/Novus Biologicals
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rat monoclonal primary antibodies to lcn2 - by Bioz Stars, 2026-03
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primary antibodies against lcn2 a2092  (ABclonal Biotechnology)
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ABclonal Biotechnology primary antibodies against lcn2 a2092
Hemosiderin deposits in ovarian tissues. Formalin-fixated paraffin-embedded ovarian tissues section of wild type (WT, n=8) and Esr1 -deficient (n=8) animals were used for staining. Serial tissue slices of WT (A) and Esr1 -deficient (B) ovaries were stained with Hematoxylin-Eosin (HE) or Perls Prussian Blue (PPB) to detect iron in form of hemosiderin. Hemosiderin can be seen in HE staining as brown-gold deposits and turns blue with PPB. The scale bars equal to 250 µm (solid boarder) or 50 µm (dashed border). Hemosiderin accumulations were found in ovarian stroma of Esr1 -deficient animals but not in WTs and were localized around hemorrhagic cysts (C) . The scale bars equal to 100 µm. (D) Immunohistochemical localization of <t>lipocalin</t> <t>2</t> <t>(LCN2)</t> was combined with PPB staining to examine whether iron-positive cells co-localize with LCN2-positive cells. WT (n=3) and Esr1 -deficient (n=4) ovaries were stained, showing no co-localization of iron and LCN2. Normal goat IgG was used as a negative control instead of the primary antibody. The scale bars equal to 500 µm (solid boarder) or 50 µm (dotted boarder and dashed border).
Primary Antibodies Against Lcn2 A2092, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/primary antibodies against lcn2 a2092/product/ABclonal Biotechnology
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Hemosiderin deposits in ovarian tissues. Formalin-fixated paraffin-embedded ovarian tissues section of wild type (WT, n=8) and Esr1 -deficient (n=8) animals were used for staining. Serial tissue slices of WT (A) and Esr1 -deficient (B) ovaries were stained with Hematoxylin-Eosin (HE) or Perls Prussian Blue (PPB) to detect iron in form of hemosiderin. Hemosiderin can be seen in HE staining as brown-gold deposits and turns blue with PPB. The scale bars equal to 250 µm (solid boarder) or 50 µm (dashed border). Hemosiderin accumulations were found in ovarian stroma of Esr1 -deficient animals but not in WTs and were localized around hemorrhagic cysts (C) . The scale bars equal to 100 µm. (D) Immunohistochemical localization of lipocalin 2 (LCN2) was combined with PPB staining to examine whether iron-positive cells co-localize with LCN2-positive cells. WT (n=3) and Esr1 -deficient (n=4) ovaries were stained, showing no co-localization of iron and LCN2. Normal goat IgG was used as a negative control instead of the primary antibody. The scale bars equal to 500 µm (solid boarder) or 50 µm (dotted boarder and dashed border).

Journal: Frontiers in Endocrinology

Article Title: Ovaries of estrogen receptor 1-deficient mice show iron overload and signs of aging

doi: 10.3389/fendo.2024.1325386

Figure Lengend Snippet: Hemosiderin deposits in ovarian tissues. Formalin-fixated paraffin-embedded ovarian tissues section of wild type (WT, n=8) and Esr1 -deficient (n=8) animals were used for staining. Serial tissue slices of WT (A) and Esr1 -deficient (B) ovaries were stained with Hematoxylin-Eosin (HE) or Perls Prussian Blue (PPB) to detect iron in form of hemosiderin. Hemosiderin can be seen in HE staining as brown-gold deposits and turns blue with PPB. The scale bars equal to 250 µm (solid boarder) or 50 µm (dashed border). Hemosiderin accumulations were found in ovarian stroma of Esr1 -deficient animals but not in WTs and were localized around hemorrhagic cysts (C) . The scale bars equal to 100 µm. (D) Immunohistochemical localization of lipocalin 2 (LCN2) was combined with PPB staining to examine whether iron-positive cells co-localize with LCN2-positive cells. WT (n=3) and Esr1 -deficient (n=4) ovaries were stained, showing no co-localization of iron and LCN2. Normal goat IgG was used as a negative control instead of the primary antibody. The scale bars equal to 500 µm (solid boarder) or 50 µm (dotted boarder and dashed border).

Article Snippet: To block non-specific antibody binding sites, tissue sections were incubated in 5% normal rabbit serum (#X0902, Agilent Technologies) in blocking solution (1% BSA, 0.1% cold fish gelatin, 0.1% Triton-X-100, 0.05% Tween ® 20 in PBS) for 90 min. Primary anti-LCN2 Antibody (#AF3508, R&D Systems, Minneapolis, MN, USA) was diluted 1:40 in blocking solution and incubated on slides at 4°C overnight.

Techniques: Staining, Immunohistochemical staining, Negative Control

Macrophages in ovarian tissues. Wild type (WT) and Esr1 -deficient ovarian tissues were either used for mRNA (WT, n=7; Esr1 –/– , n=5-6), protein analysis (WT, n=3; Esr1 –/– , n=3) or immunofluorescence staining (WT, n=3; Esr1 –/– , n=3). (A) Relative mRNA expression of pan-macrophage markers Cd68 and Adgre were detected by RT-qPCR. (B) Western blot analysis was used to detect CD68 protein expression, which was quantified densitometrically and plotted relative to β-actin expression. F4/80 (green) and LCN2 (red) protein expression was visualized by immunofluorescence staining with nuclear DAPI counterstaining in (C) WT and Esr1 -deficient (D) ovaries. (E) Normal rat and goat IgG were used as a negative control instead of the primary antibodies. The scale bars equal to 100 µm (solid boarder) or 50 µm (dashed border). (F) Relative mRNA expression of Nos2 and Il1r1 , as well as Arg1 , Cd163 and Mrc (G) were determined by RT-qPCR. All data (A-B, F-G) are displayed as mean ± SD. For statistical analysis, Students t -test was done. Significant differences between groups are marked with asterisks: * p <0.05, ** p <0.01, *** p <0.001, ns =not significant.

Journal: Frontiers in Endocrinology

Article Title: Ovaries of estrogen receptor 1-deficient mice show iron overload and signs of aging

doi: 10.3389/fendo.2024.1325386

Figure Lengend Snippet: Macrophages in ovarian tissues. Wild type (WT) and Esr1 -deficient ovarian tissues were either used for mRNA (WT, n=7; Esr1 –/– , n=5-6), protein analysis (WT, n=3; Esr1 –/– , n=3) or immunofluorescence staining (WT, n=3; Esr1 –/– , n=3). (A) Relative mRNA expression of pan-macrophage markers Cd68 and Adgre were detected by RT-qPCR. (B) Western blot analysis was used to detect CD68 protein expression, which was quantified densitometrically and plotted relative to β-actin expression. F4/80 (green) and LCN2 (red) protein expression was visualized by immunofluorescence staining with nuclear DAPI counterstaining in (C) WT and Esr1 -deficient (D) ovaries. (E) Normal rat and goat IgG were used as a negative control instead of the primary antibodies. The scale bars equal to 100 µm (solid boarder) or 50 µm (dashed border). (F) Relative mRNA expression of Nos2 and Il1r1 , as well as Arg1 , Cd163 and Mrc (G) were determined by RT-qPCR. All data (A-B, F-G) are displayed as mean ± SD. For statistical analysis, Students t -test was done. Significant differences between groups are marked with asterisks: * p <0.05, ** p <0.01, *** p <0.001, ns =not significant.

Article Snippet: To block non-specific antibody binding sites, tissue sections were incubated in 5% normal rabbit serum (#X0902, Agilent Technologies) in blocking solution (1% BSA, 0.1% cold fish gelatin, 0.1% Triton-X-100, 0.05% Tween ® 20 in PBS) for 90 min. Primary anti-LCN2 Antibody (#AF3508, R&D Systems, Minneapolis, MN, USA) was diluted 1:40 in blocking solution and incubated on slides at 4°C overnight.

Techniques: Immunofluorescence, Staining, Expressing, Quantitative RT-PCR, Western Blot, Negative Control

Journal: Frontiers in Endocrinology

Article Title: Ovaries of estrogen receptor 1-deficient mice show iron overload and signs of aging

doi: 10.3389/fendo.2024.1325386

Figure Lengend Snippet:

Article Snippet: To block non-specific antibody binding sites, tissue sections were incubated in 5% normal rabbit serum (#X0902, Agilent Technologies) in blocking solution (1% BSA, 0.1% cold fish gelatin, 0.1% Triton-X-100, 0.05% Tween ® 20 in PBS) for 90 min. Primary anti-LCN2 Antibody (#AF3508, R&D Systems, Minneapolis, MN, USA) was diluted 1:40 in blocking solution and incubated on slides at 4°C overnight.

Techniques: Clinical Proteomics, Mass Spectrometry, Saline, Reverse Transcription, Real-time Polymerase Chain Reaction